Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid

Liquid-liquid extraction and solid phase followed by high-performance liquid chromatography (HPLC) connected with ultraviolet (UV) detection were used for the determination of phytohormones. The parameters influencing performance HPLC-UV method, including composition mobile gradient elution, column temperature, flow rate, wavelength, optimized. This method can simultaneously determine 11 phytohormones, nine cytokinins, indole-3-acetic acid, abscisic acid. limit this is 0.22 to 1.1 µg L?1, coefficient factors linear regression are >0.998. recoveries target phytohormones ranged between 62.1~109.4%, relative standard deviations <10%. suitable determining especially in young panicles, roots, xylem sap rice plants.